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Anti-TMT antibody-conjugated resin-based enrichment To enrich S-glutathionylated peptides, tryptic peptides were dissolved in NETN buffer (100 mM NaCl, 1 mM EDTA, 50 mM Tris-HCl, 0.5% NP-40, pH 8.0) and incubated with pre-washed anti-TMT antibody resins (Prod#90076, ThermoFisher Scientific) at 4C overnight with gentle shaking in the dark

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Madwani K, Huang HJ, Shelton DN, Fu S, Tsimberidou AM, Piha-Paul SA, Naing A, Hong DS, Karp DD, Andrews DL, Cabrilo G, Kopetz ES , Luthra R, Kee BK, Eng C, Morris VK, Karlin-Neumann GA, Meric-Bernstam F, Janku F

This unique infusion combines NAD+ with the multi-beneficial nutrients of The Works (glutathione)